Author(s)

Maria Eugênia Lisei-de-Sá^1,2*, Fabricio B. M. Arraes^1,3*, Giovani G. Brito⁴, Magda A. Beneventi^1,3, Isabela T. Lourenço-Tessutti¹, Angelina M. M. Basso^1,5, Regina M. S. Amorim¹, Maria C. M. Silva¹, Muhammad Faheem¹, Nelson G. Oliveira⁶, Junya Mizoi⁷, Kazuko Yamaguchi-Shinozaki⁷, Maria Fatima Grossi-de-Sa^1,8*

¹Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil.
²Empresa de Pesquisa Agropecuária de Minas Gerais, Uberaba, Brazil.
³Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
⁴Embrapa Clima Temperado, Pelotas, Brazil.
⁵Universidade de Brasília, Brasília, Brazil.
⁶Embrapa Agroenergia, Brasília, Brazil.
⁷Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Japan.
⁸Universidade Católica de Brasília, Brasília, Brazil.

Drought is a major environmental factor limiting cotton (Gossypium hirsutum L.) productivity worldwide and projected climate changes could increase their negative effects in the future. Thus, targeting the molecular mechanisms correlated with drought tolerance without reducing productivity is a challenge for plant breeding. In this way, we evaluated the effects of water deficit progress on AtDREB2A-CA transgenic cotton plant responses, driven by the stress-inducible rd29 promoter. Besides shoot and root morphometric traits, gas exchange and osmotic adjustment analyses were also included. Here, we present how altered root traits shown by transgenic plants impacted on physiological acclimation responses when submitted to severe water stress. The integration of AtDREB2A-CA into the cotton genome increased total root volume, surface area and total root length, without negatively affecting shoot morphometric growth parameters and nor phenotypic evaluated traits. Additionally, when compared to wild-type plants, transgenic plants (17-T₀ plants and its progeny) highlighted a gradual pattern of phenotypic plasticity tosome photosynthetic parameters such as photosynthetic rate and stomatal conductance with water deficit progress. Transgene also promoted greater shoot development and root robustness (greater and deeper root mass) allowing roots to grow into deeper soil layers. The same morpho-physiological trend was observed in the subsequent generation (17.6-T₂). Our results suggest that the altered root traits shown by transgenic plants are the major contributors to higher tolerance response, allowing the AtDRE2A-CA-cotton plants to maintain elevated stomatal conductance and assimilate rates and, consequently, reducing their metabolic costs involved in the antioxidant responses activation. These results also suggest that these morpho-physiological changes increased the number of reproductive structures retained per plant (26% higher) when compared with its non-transgenic counterpart. This is the first report of cotton plants overexpressing the AtDRE2A-CA transcription factor, demonstrating a morpho-physiological and yield advantages under drought stress, without displaying any yield penalty under irrigated conditions. The mechanisms by which the root traits influenced the acclimation of the transgenic plants to severe water deficit conditions are also discussed. These data present an opportunity to use this strategy in cotton breeding programs in order to improve drought adaptation toward better rooting features.

Dehydration Responsive Element Binding Factors, Water Deficit Tolerance, Gossypium hirsutum, Physiological Phenotyping, Transcription Factor, Stress-Inducible Promoter

Lisei-de-Sá, M. , M. Arraes, F. , Brito, G. , Beneventi, M. , Lourenço-Tessutti, I. , Basso, A. , Amorim, R. , M. Silva, M. , Faheem, M. , Oliveira, N. , Mizoi, J. , Yamaguchi-Shinozaki, K. and Grossi-de-Sa, M. (2017) AtDREB2A-CA Influences Root Architecture and Increases Drought Tolerance in Transgenic Cotton. Agricultural Sciences, 8, 1195-1225. doi: 10.4236/as.2017.810087.