Author(s)

Atsuko Fujita¹, Takaya Nakata², Makoto Umeda², Hiroaki Masuzaki³, Hirofumi Sawai^4*

¹Department of Periodontology, Graduate School of Dentistry, Osaka Dental University, Osaka, Japan.
²Department of Periodontology, Osaka Dental University, Osaka, Japan.
³Division of Endocrinology, Diabetes and Metabolism, Hematology, Rheumatology, Graduate School of Internal Medicine, University of the Ryukyus, Okinawa, Japan.
⁴Department of Internal Medicine, Osaka Dental University, Osaka, Japan.

It has been proposed that 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), which activates glucocorticoids, plays a role in chronic inflammatory diseases including metabolic diseases, rheumatoid arthritis, and ulcerative colitis. We have recently reported that the expression of 11β-HSD1 is increased in the gingiva of patients with chronic periodontitis and in that of rats with ligature-induced periodontitis. In this study, to further demonstrate the involvement of 11β-HSD1 in chronic periodontitis, the expression of 11β-HSD1 was investigated in another rat model of experimental periodontitis induced by intragingival injection of lipopolysaccharide from Porphyromonas gingivalis (LPS-PG). Alveolar bone loss was observed two weeks after intragingival injection of LPS-PG. The level of 11β-HSD1 mRNA assessed by real-time reverse transcriptase-polymerase chain reaction was significantly elevated in LPS-PG-induced periodontitis compared with controls. The expression of 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), which inactivates glucocorticoids, was not significantly different between control and LPS-PG-induced periodontitis. The expression of 11β-HSD1 was significantly correlated with that of TNF in LPS-PG-induced periodontitis. The increased expression of 11β-HSD1 protein in LPS-PG-induced periodontitis was confirmed by immunohistochemistry using anti-11β-HSD1 antibody. These results further suggest a role for 11β-HSD1 in the pathogenesis of chronic periodontitis.

Chronic Periodontitis, 11β-Hydroxysteroid Dehydrogenase Type 1, Lipopolysaccharide, Porphyromonas gingivalis

Fujita, A. , Nakata, T. , Umeda, M. , Masuzaki, H. and Sawai, H. (2017) Increased Expression of 11β-Hydroxysteroid Dehydrogenase Type 1 in Experimental Periodontitis Induced by Lipopolysaccharide from Porphyromonas gingivalis. Open Journal of Stomatology, 7, 429-438. doi: 10.4236/ojst.2017.710037.