Greenhouse is an important place for crop growth, and it is necessary to control the temperature of growing environment in winter. In addition, the root temperature underground also plays a decisive role for plants growth. Adopting underground heating to increase the temperature can effectively improve the yield of crops. The objective of our study was to model the heat transfer of greenhouse underfloor heating which is analyzed and simplified based on the FLUENT software by changing the several important factors that affect the temperature distribution: pipe diameter, pipe spacing, laying depth, supplied water temperature and flow rate, as boundary conditions to simulate the changes of the soil temperature field around the winter night environment. Researching the temperature distribution of the greenhouse, the soil surface and the plant root layer under the different parameters and the basic rules of the heating system are summarized. The results show that the water supply temperature, pipe spacing and diameter of the pipe has a greater impact on the ground and room temperature, and the laying depth has greater impact on the temperature uniformity of the ground, the velocity of water in pipe has little impact on the uniformity of ground temperature.