In Vitro Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank - American Journal of Plant Sciences

AJPS > Vol.8 No.9, August 2017

In Vitro Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank ()

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Download as PDF (Size: 1166KB) PP. 2238-2254

DOI: 10.4236/ajps.2017.89150 1,170 Downloads 3,400 Views Citations

Author(s)

Analí Lizárraga¹, Marga Fraga², Javier Ascasíbar³, María Luz González¹

Affiliation(s)

¹Department of Plant Physiology, University of Santiago de Compostela, Santiago de Compostela, Spain.
²Cultigar, Brión, Spain.
³Centro de Investigaciones Agrarias de Mabegondo INGACAL-CIAM, Abegondo, Spain.

ABSTRACT

Eight apple (Malus x domestica) and two pear (Pyrus communis) traditional cultivars of commercial interest were selected from the germplasm bank CIAM (Centro de Investigaciones Agrarias de Mabegondo, NW of Spain) one of the most important collections of The Iberian Peninsula. In order to define the establishment protocol, apical buds 1.0 - 1.5 millimetre length of the selected cultivars were established in solid mineral medium Murashige and Skoog (MS) with modified vitamins (thiamine 10X) and supplemented with 1 mg·L^-1 6-benzyladenine (BA), 0.2 mg·L^-¹ gibberellic acid (GA₃) and 0.3 mg·L^-¹ of indole-3-butyric acid (IBA). The highest shoot initiation rates were achieved with apple cultivar “Principe Grande” (55%) and “Barburiña” pear cultivar (50%); the lowest rate was obtained with apple cultivar “Camoesa” (11%). Initiation rates for shoots culture varied between 22% and 37% for the other cultivars. The cytokinins BA, zeatin, 2-isopentyladenine (2iP) and thidiazuron (TDZ) were tested at concentrations of 0, 0.25, 0.5 and 1 mg·L^-¹ in the absence or presence of IBA (0.1 mg·L^-¹) for the multiplication step. For all cultivars, the highest multiplication rates were obtained with medium supplemented with TDZ and also in media containing BA. The in vitro rooting success of shoots in medium with 0.1 mg·L^-¹ IBA without cytokinins, and the survival rate of plantlets acclimatized under greenhouse conditions depended on the genotype of the different cultivars. In vitro propagation of these ten traditional apple and pear cultivars in NW Spain has not previously been reported.

KEYWORDS

Apple Shoots, Pear Shoots, Axillary Bud, Multiplication, Cytokinin, Gene Bank

Share and Cite:

Lizárraga, A. , Fraga, M. , Ascasíbar, J. and González, M. (2017) In Vitro Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank. American Journal of Plant Sciences, 8, 2238-2254. doi: 10.4236/ajps.2017.89150.

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