Author(s)

Brahim El Bouzdoudi^1*, Rabah Saïdi², Zineb Nejjar El Ansari¹, Mohammed L’bachir El Kbiach¹, Patrick Martin³, Alain Badoc⁴, Ahmed Lamarti¹

¹Laboratory of Plant Biotechnology, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco.
²Department of Matter and Life Sciences, High Normal School, Martil, Morocco.
³Chemistry Department, IUT Béthune, F-62408, Artois University, Béthune, France.
⁴Axe MIB (Molécules d’Intérêt Biologique), Unité de Recherche Enologie EA 4577, USC 1366 INRA, UFR des Sciences.

Adventitious budding from embryonic cotyledons of immature seeds of carob was obtained. The combination of BAP (4.44 μM) and NAA (1.5 μM) furthered the neoformation of adventitious buds. These latter were multiplied on MS medium added with BAP (2.22 μM). Stems and leaves growing were improved by adding 2.02 μM GA₃. Elongation was favored by 0.5 μM NAA. 70% of rooting was obtained with 10 μM IBA.

Ceratonia siliqua L., Micropropagation, Adventitious Bud, Embryonic Cotyledons

Bouzdoudi, B. , Saïdi, R. , El Ansari, Z. , El Kbiach, M. , Martin, P. , Badoc, A. and Lamarti, A. (2017) Micropropagation of Carob (Ceratonia silique L.) through Adventitious Buds of Immature Embryonic Cotyledons. American Journal of Plant Sciences, 8, 2180-2195. doi: 10.4236/ajps.2017.89146.