Callogenesis of Cork Oak (Quercus suber L.) through In Vitro Culture of Nodes and Internodes - American Journal of Plant Sciences

AJPS > Vol.8 No.8, July 2017

Callogenesis of Cork Oak (Quercus suber L.) through In Vitro Culture of Nodes and Internodes ()

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DOI: 10.4236/ajps.2017.88123 1,201 Downloads 2,584 Views Citations

Author(s)

Mohammed L’bachir El Kbiach¹, Brahim El Bouzdoudi^1*, Rabah Saïdi², Zineb Nejjar El Ansari¹, Safaa Rahmouni¹, Ahmed Lamarti¹

Affiliation(s)

¹Laboratory of Plant Biotechnology, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco.
²Department of Matter and Life Sciences, High Normal School, Martil, Morocco.

ABSTRACT

The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting; small white embryos appear on the surface of calluses.

KEYWORDS

Cork oak, Quercus suber L., Nodes, Internodes, Callogenesis

Share and Cite:

Kbiach, M. , Bouzdoudi, B. , Saïdi, R. , Ansari, Z. , Rahmouni, S. and Lamarti, A. (2017) Callogenesis of Cork Oak (Quercus suber L.) through In Vitro Culture of Nodes and Internodes. American Journal of Plant Sciences, 8, 1801-1819. doi: 10.4236/ajps.2017.88123.

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