Author(s)

Soo-Seong Lee^1*, Byoung Ho Hwang¹, Tae Yoon Kim¹, Jeongmin Yang², Na Rae Han², Jongkee Kim², Hyun Hee Kim³, Hadassah Roa Belandres³

¹BioBreeding Institute, Sillyeong-Ro 153, Daeduk, Ansung, Gyeonggi, Korea.
²Dept. of Integrative Plant Science, Chung-Ang University, Ansung, Korea.
³Plant Biotechnology Institute, Department of Life Science, Sahmyook University, Seoul, Korea.

A stable progeny was developed through induced mutation, using microspore culture, of the hybrid (F₁F₁) produced by crossing a newly synthesized, unstable allopolyploid (F₁) and a stable cultivar, BB#1(F₁) in xBrassicoraphanus. An F₁F₁ plant was subjected to the induced mutation system established during production of BB#1. Morphological characteristics of the progeny such as color, and leaf number and length, differed from those of BB#1. The bolting time of the progeny in spring cropping was very late compared to BB#1, allowing it to be grown to an adult plant in spring. Genomic in situ hybridization analysis of pollen mother cells at prophase I identified 19 bivalents, 10 from Brassica rapa and 9 from Raphanus sativus. The glucoraphenin content was almost identical to that of BB#1. Two cultivars are available in the baemoochae crop now. These results indicate that induced mutation using microspore culture is a viable method of stabilizing intergeneric allopolyploids between B. rapa and R. sativus.

xBrassicoraphanus, Intergeneric Hybrid, Baemoochae, Brassica rapa, Raphanus sativus

Lee, S. , Hwang, B. , Kim, T. , Yang, J. , Han, N. , Kim, J. , Kim, H. and Belandres, H. (2017) Developing Stable Cultivar through Microspore Mutagenesis in ×Brassicoraphanus koranhort, Inter-Generic Allopolyploid between Brassica rapa and Raphanus sativus. American Journal of Plant Sciences, 8, 1345-1356. doi: 10.4236/ajps.2017.86091.