Author(s)

Hamdia M. Abd El-Samad, D. Mostafa, Kholoud N. Abd El-Hakeem

Botany Department, Faculty of Science, Minia University, El-Minia, Egypt.

The response of wheat plants to different osmotic stress levels varied among the different organs root, shoot and spike and the situation of these organs with application of two Cu⁺⁺ levels 5 mM and 25 mM as CuSO₄. The sensitivity of root organ was related with reduction in fresh, dry matter and length. This resulted from reduction of soluble sugar reflected a reduction in water uptake and K⁺ content of the cell sap. In the moderate organ spike, the reduction in fresh, dry matter and length were concomitant with the accumulation of soluble sugar and a huge accumulation of soluble protein. In the higher organ shoot, this related with more water uptake which in turn induced an accumulation of soluble protein and cofactor K⁺ content. It can be recorded that shoot was higher Na⁺ accumulation than root and spike. Data also showed further stimulatory effect on growth parameters by Cu⁺⁺ applications with either concentration (7.5 mM and 25 mM). Irrigating the soil with either 7.5 or 25 mM CuSO₄ induced a huge accumulation in soluble sugar, soluble protein and nitrate reductase. Cupper treatment with either concentration 7.5 mM or 25 mM induced a marked decrease in Na⁺ content at all OSL and has no significant change in the accumulation of K⁺ in both shoot and spike whereas induced a huge accumulation in root organ. The synthesis of protein bands with molecular weight 32.3 KDa at -1.5 MPa NaCl level treated with either 7.5 mM or 25 mM Cu⁺⁺ concentrations was induced. Also the appearance of protein band with molecular weight 37KDa induced only at Cu⁺⁺ treatments with 25 mM concentration in both control and under different osmotic levels (0.0, -0.3 MPa, -0.9 MPa, -1.5 MPa NaCl).

Osmotic Stress Levels, Cupper, Tolerance, Wheat, Protein Bands

El-Samad, H. , Mostafa, D. and El-Hakeem, K. (2017) The Combined Action Strategy of Two Stresses, Salinity and Cu++ on Growth, Metabolites and Protein Pattern of Wheat Plant. American Journal of Plant Sciences, 8, 625-643. doi: 10.4236/ajps.2017.83043.