Author(s)

Azizul Isha¹, Nur A’thifah Yusof¹, Intan Safinar Ismail^1,2*

¹Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia.
²Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia.

In this study, Fourier Transform Infrared (FTIR)-, ¹H Nuclear Magnetic Resonance (NMR)-spectroscopy and multivariate statistical analysis were used for the screening of in vitro anti-inflammatory activity on three different germplasm accessions namely 11,341 (P), 11,248 (T) and 11,265 (H) of Malaysian Andrographis paniculata (A. paniculata) leaf. The anti-inflammatory activity by nitric oxide (NO) inhibition assay in relation to the different harvesting ages and times of A. paniculata leaf was determined through comparison by partial least-squares analysis (PLS) using SIMCA-P. The PLS allowed the separation and correlation between the NO assay with the phytochemical present due to the effects of different harvesting ages and times. From the PLS plots, conclusions were drawn with respect to the correlation between A. paniculata leaf metabolites with the anti-inflammatory results which showed that 180 days after transplanting (DAT) of morning session for accessions T and H, and evening for P gave the highest anti-inflammatory activity.

Andrographis paniculata, Anti-Inflammatory, NO Inhibition, Partial Least-Squares Analysis, FTIR, 1H NMR

Isha, A. , Yusof, N. and Ismail, I. (2016) Harvesting Age and Time Effect of Andrographis paniculata Leaf on Its Anti-Inflammatory Activity. Journal of Biosciences and Medicines, 4, 175-188. doi: 10.4236/jbm.2016.412021.