Detection of Antibodies against Mycoplasma mycoides subsp. capri in Goats with the Complement Fixation Test - Advances in Microbiology

AiM > Vol.6 No.13, November 2016

Detection of Antibodies against Mycoplasma mycoides subsp. capri in Goats with the Complement Fixation Test ()

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DOI: 10.4236/aim.2016.613090 1,347 Downloads 2,135 Views Citations

Author(s)

José L. Corona-Vargas¹, Myrna A. Vicencio-Mallén¹, Frida Salmerón-Sosa¹, Erika M. Carrillo-Casas², Francisco J. Trigo-Tavera³, Rosa E. Miranda-Morales^1*

Affiliation(s)

¹Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, Coyoacán, México.
²Departamento de Biología Molecular e Histocompatibilidad, Dirección de Investigación, Hospital General Dr. Manuel Gea González, Calz, Tlalpan, México.
³Departamento de Patología, Facultad de Medicina Veterinaria y Zootecnia,Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, Coyoacán, México.

ABSTRACT

Mycoplasma mycoides subsp. capri is the causative agent of severe and acute respiratory problems in goats, which spreads rapidly and represents high mortality. The serological profile of the goat population, from nine regions in seven states of Mexico, was screened by the Complement Fixation test (CF) in sera from asymptomatic goats and animals with mild respiratory symptoms. Sera and nasal swabs of 827 goats were collected for the isolation of the organism. An antiserum was prepared against a previously isolated field strain of Mycoplasma mycoides subsp. capri. CF Antibody titers were associated with the results of the isolates to determine the cutoff point. The CF was considered as positive if its result was ≥1/16. The CF registered 251 positive goats (30.35%) and 576 (69.65%) negative; the test showed high sensitivity (93.33%) and specificity (72.27%). In the specific case of diagnosis for mycoplasmosis associated with respiratory problems in goats, the CF proved to be a good diagnosis test, this study determined that 30% of the goat population showed antibody titers against Mycoplasma mycoides subsp. capri and revealed those animals who have had contact with this microorganism during their lives regardless of the presence or absence of respiratory symptoms.

KEYWORDS

Complement Fixation, Indirect Agglutination, Mycoplasmosis, Mycoplasma mycoides, Mycoplasma mycoides subsp. capri

Share and Cite:

Corona-Vargas, J. , Vicencio-Mallén, M. , Salmerón-Sosa, F. , Carrillo-Casas, E. , Trigo-Tavera, F. and Miranda-Morales, R. (2016) Detection of Antibodies against Mycoplasma mycoides subsp. capri in Goats with the Complement Fixation Test. Advances in Microbiology, 6, 959-964. doi: 10.4236/aim.2016.613090.

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