ABSTRACT
Detection of F. solani f. sp. cucurbitae causal agent of the crown and root rot disease
of melon race 1, race 2 is difficult. It is based only on morphological characteristic. In
this study, forty isolates identified as Fusarium solani based on morphological characterization, F. solani was one of the most frequently isolated species. Molecular
identification of these isolates by PCR technique using species-specific primer, indicated
that thirty-two isolates, amplified product 580 bp (race 1) and two isolate amplified
product 580 bp (race 2), while six isolates were not amplified with primer of
both races. Production of Trichothecenes (T2-toxen, DON.) by Fusarium solani was
conducted on isolates confirmed as belonging in the F. solani by PCR. The results
indicated that the presence of Tri5, Tri13 genes is coding the ability of synthesis mycotoxin. In vitro, the results indicated that NPs (AgNPs, MgNPs) and chemical
(Phylex) possess the antifungal properties against at various level. Treatment with
(AgNPs 150 ppm, MgNPs 2%, 3% ppm) and 3% Phylex resulted in maximum inhabitation
of F. solani . In vivo, five characters (height plant, hoot ant root fresh and dry
weight) were measured based on the greenhouse, field experimental results. Treatment
with (AgNPs, MgNPs) and Phylex had higher measured parameters than positive
control.