Hippocampus, an area of cortex that plays an important role in thinking, planning and remembering. In Alzheimer’s disease (AD), the hippocampus is one of the first areas of the brain to become shriveled and this leads to the memory less, damage in learning and declaration of emotional behaviors. In this paper, we investigate the effects of sex on hippocampus gray matter (HGM) atrophy in four groups of participants, namely, males with AD (M-AD, n = 34), age-matched normal male controls (M-NC, n = 34), females with AD (F-AD, n = 34), and age matched normal female controls (F-NC, n = 34) from ADNI dataset. In this regard, Analysis of variance (ANOVA) is employed to compare means of HGM differences among groups. The statistical results obtained by ANOVA show that the distribution of HGM atrophy is effected by sex. Also there was a significant diagnosis with higher severity in the F-AD compared to M-AD. The AD studies based on the sex may help to figure out the root of AD mechanisms and poten-tially can be used as an imaging marker for the studies of AD in the future.