mRNA of Expression of Per 1 in Mice Bone Marrow Mesenchymal Stem Cells Irradiated by Red Laser - Optics and Photonics Journal

OPJ > Vol.6 No.8, August 2016

mRNA of Expression of Per 1 in Mice Bone Marrow Mesenchymal Stem Cells Irradiated by Red Laser ()

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DOI: 10.4236/opj.2016.68B033 1,564 Downloads 2,185 Views

Author(s)

Fei Peng

Affiliation(s)

Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.

ABSTRACT

A core group of circadian genes regulate the circadian rhythms in mammalian cells. However, the mammalian cellular circadian rhythm in photobiomodulation remains unknown. A lot of evidence has shown that >20% of expressed mRNAs in bone stissues oscillate in a circadian manner. The aim of this paper is to investigate the mRNA expression of period 1 (per 1) in murine bone marrow-derived mesenchymal stem cells (BMSCs) which were irradiated by 635 nm red laser light. The cells were seeded in 35mm tissue-culture dishes at a density of 8 × 104 cells/dish and cultured in Dulbecco’s modi?ed Eagle’s medium (DMEM). BMSCs were irradiated once by 635 nm red light with radiation energies 0 J/cm2, 1 J/cm2, 4 J/cm2. mRNA expression of per 1 via Semi-Quantitative Real-time RT-PCR at 0h, 6 h, 12 h, 18 h, 24 h. The genes displayed a oscillatory period of nearly 24 hours. And 635 m laser light changed the mRNA expression of per 1. We conclude that red light irradiation can affect the circadian rhythm of BMSCs.

KEYWORDS

Photobiostimulation, Mesenchymal Stem Cells (MSCs), Period 1, Circadian Rhythm

Share and Cite:

Peng, F. (2016) mRNA of Expression of Per 1 in Mice Bone Marrow Mesenchymal Stem Cells Irradiated by Red Laser. Optics and Photonics Journal, 6, 201-204. doi: 10.4236/opj.2016.68B033.

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