Effects of Cinnamaldehyde, Ocimene, Camphene, Curcumin and Farnesene on Candida albicans - Advances in Microbiology

AiM > Vol.6 No.9, August 2016

Effects of Cinnamaldehyde, Ocimene, Camphene, Curcumin and Farnesene on Candida albicans ()

HTML XML

Download as PDF (Size: 4343KB) PP. 627-643

DOI: 10.4236/aim.2016.69062 2,256 Downloads 4,576 Views Citations

Author(s)

Archana D. Thakre¹, Shradha V. Mulange¹, Santosh S. Kodgire¹, Gajanan B. Zore^1,2, S. Mohan Karuppayil^1*

Affiliation(s)

¹School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, India.
²Department of Crops and Soil Sciences, Washington State University, Pullman, USA.

ABSTRACT

Efficacy of five plant molecules against thirty three clinical isolates and two standard strains of C. albicans, differentially susceptible to fluconazole (FLC) is tested in this study. Effect on biofilm (adhesion, development and maturation) formation, morphogenesis and synergy with fluconazole (FLC) against a FLC resistant strain of Candida albicans ATCC 10231 is also evaluated. All the plant molecules tested were equally effective against isolates and strains of C. albicans (N = 35) tested in this study. Cinnamaldehyde was found most effective against planktonic growth followed by ocimene. Both the molecules exhibited fungicidal activity and killed 99.9% of inoculum within 80 and 20 min of exposure respectively at 0.62 mM and 176.8 mM concentrations. Curcumin (5 - 20 mM), camphene (8 - 32 mM) and farnesene (25 - 100 mM), although inhibited planktonic growth, were fungistatic. All the five plant molecules tested in this study inhibited morphogenesis significantly and exhibited considerable activity against biofilm formation. Inhibition of biofilm was found to be stage specific i.e. efficacy was more against adhesion followed by developing and mature biofilm. Plant molecules tested exhibited excellent synergy with fluconazole. However FIC index values 0.155, 0.062 and 0.046 indicate that ocimene was the most effective synergistic molecule inhibited planktonic growth, developing biofilm and mature biofilm growth respectively at very low concentrations. This is the first report of anti-Candida activity of three terpenoids viz. ocimene, farnesene and camphene against planktonic & biofilm growth, morphogenesis as well as synergy with FLC. Plant molecules tested in this study may find use in antifungal chemotherapy individually and or in a combination with FLC.

KEYWORDS

Ocimene, Farnesene, Camphene, Synergy, Candida albicans, Biofilm, Morphogenesis

Share and Cite:

Thakre, A. , Mulange, S. , Kodgire, S. , Zore, G. and Karuppayil, S. (2016) Effects of Cinnamaldehyde, Ocimene, Camphene, Curcumin and Farnesene on Candida albicans. Advances in Microbiology, 6, 627-643. doi: 10.4236/aim.2016.69062.

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies