Comparative Study of Two Conventional Methods Used for Coliform Enumeration from Port Harcourt Waters - Open Access Library Journal

OALibJ > Vol.3 No.3, March 2016

Comparative Study of Two Conventional Methods Used for Coliform Enumeration from Port Harcourt Waters ()

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DOI: 10.4236/oalib.1102500 3,528 Downloads 6,021 Views Citations

Author(s)

Prince Chinonso Nnadozie

Affiliation(s)

Department of Microbiology, Faculty of Sciences, University of Port Harcourt, Port Harcourt, Nigeria.

ABSTRACT

This study compares two conventional methods: Membrane Filtration (MF) and Multiple Tube Fermentation (MTF) used in the analysis of two Port Harcourt natural water sources, Abonnema Wharf (A) and Tourist Beach (B). However, these methods are generally used in the determination of the sanitary condition of the waters and its suitability for general use. It is observed from the study that the MF gives a more reliable and precised data than that of the MTF which is time consuming, labour intensive and less precised. Bacterial isolates such as the Enterococcus faecalis not present with the use of MF method may be as a result of seasonal changes, spatial distribution of organism, and varying degree of human/animal and industrial activities/influence on the ecosystem. Studies on the effects of physiological stress especially stress by chlorination should be carried out on the waters in order to substantiate the effectiveness of each method and also identify the best conventional method to be applied during analysis of water.

KEYWORDS

Membrane Filtration, Most Probable Number, Coliforms

Share and Cite:

Nnadozie, P. (2016) Comparative Study of Two Conventional Methods Used for Coliform Enumeration from Port Harcourt Waters. Open Access Library Journal, 3, 1-5. doi: 10.4236/oalib.1102500.

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