Protective Effect of bFGF on Bone Marrow Mesenchymal Stem Cell against Hydrogen Peroxide-Induced Apoptosis - Open Access Library Journal

OALibJ > Vol.3 No.2, February 2016

Protective Effect of bFGF on Bone Marrow Mesenchymal Stem Cell against Hydrogen Peroxide-Induced Apoptosis ()

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DOI: 10.4236/oalib.1102393 766 Downloads 1,529 Views

Author(s)

Xiaoqing Gao¹, Guangqiang Hu¹, Guangbi Fan¹, Zhiqiang Mei², Li Deng¹, Jie Du^1*

Affiliation(s)

¹Department of Anatomy and Neurobiology, Sichuan Medical University, Luzhou, China.
²Preclinical Medicine Research Center, Sichuan Medical University, Luzhou, China.

ABSTRACT

Bone marrow mesenchymal stem cells (BMSCs) transplantation has emerged as a promising treatment for various central nervous system diseases. However, oxidative stress results in poor survival of transplanted BMSCs in injury central nervous system (CNS). Basic fibroblast growth factor (bFGF) has been reported to have anti-apoptotic properties in serial of cells in oxidative stress. This study was designed to investigate protective effect and potential mechanisms of bFGF against hydrogen peroxide (H₂O₂)-induced apoptosis of BMSCs. BMSCs were pretreated with 20 ng/ml of bFGF for 30 minutes, followed by exposure to 500 μM H₂O₂ and bFGF together for 1 hour. bFGF treatment significantly attenuated H₂O₂-induced cytotoxicity and apoptosis of BMSCs. Moreover, bFGF effectively decreased the levels of TNF-α and IL-6 and increased expression ratio of Bcl-2/Bax in H₂O₂-induced BMSCs. The findings suggest that bFGF is likely to be an effectively protective agent against oxidative stress-induced apoptosis for BMSCs.

KEYWORDS

Basic Fibroblast Growth Factor, Bone Marrow Mesenchymal Stem Cells, Hydrogen Peroxide, Oxidative Stress, Apoptosis, Mechanism

Share and Cite:

Gao, X. , Hu, G. , Fan, G. , Mei, Z. , Deng, L. and Du, J. (2016) Protective Effect of bFGF on Bone Marrow Mesenchymal Stem Cell against Hydrogen Peroxide-Induced Apoptosis. Open Access Library Journal, 3, 1-8. doi: 10.4236/oalib.1102393.

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