Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B - Open Access Library Journal

OALibJ > Vol.2 No.3, March 2015

Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B ()

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DOI: 10.4236/oalib.1101402 1,361 Downloads 2,037 Views Citations

Author(s)

Erick Ntambwe Kamangu^1*, Adawaye Chatte², Raphael Boreux³, Fabrice Susin⁴, Richard Lunganza Kalala¹, Georges Lelo Mvumbi¹, Patrick De Mol³, Marie-Pierre Hayette^3,4, Dolores Vaira⁴

Affiliation(s)

¹Unit of Molecular Biology, Department of Basic Sciences, Faculty of Medicine, University of Kinshasa (UNIKIN), Kinshasa, Democratic Republic of Congo (DRC).
²University Institute of Science and Technology of Abeche, Abeche, Chad.
³Laboratory of Clinical Microbiology, University Hospital-University of Liège, Liege, Belgium.
⁴AIDS Reference Laboratory (LRS), University Hospital-University of Liège, Liege, Belgium.

ABSTRACT

Context: The in-house techniques or experimental methods are increasingly recommended for their low-cost reagents for the determination of the Viral Load (VL) in resource-limited settings. The objective of this study was to compare the determination of VL from HIV-1 non-B samples by an in-house technique with the COBAS AmpliPrep/TaqMan version 2.0. Method: In this cross-sectional study, 39 plasma samples from patients infected with HIV type 1 non-B from N’Djamena and Kinshasa were used to determine the VL using the two techniques. Results: The mean values of VL are respectively 4.68 ± 1.26 and 4.58 ± 1.33 log₁₀ RNA copies/ml for the COBAS AmpliPrep/TaqMan assays and the in-house assays. A good correlation (Spearman Correlation) was obtained, with a coefficient (R²) of 0.9452. Conclusion: This study demonstrates that there is no significant difference between the results of VL determined by the COBAS AmpliPrep/TaqMan assays and the in-house assays used.

KEYWORDS

COBAS AmpliPrep/TaqMan, In-House PCR Assays, HIV-1 Non-B

Share and Cite:

Kamangu, E. , Chatte, A. , Boreux, R. , Susin, F. , Kalala, R. , Mvumbi, G. , Mol, P. , Hayette, M. and Vaira, D. (2015) Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B. Open Access Library Journal, 2, 1-7. doi: 10.4236/oalib.1101402.

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