Share This Article:

Detection and Transfer of Extended Spectrum Beta Lactamase Enzymes from Untreated Hospital Waste Water

Full-Text HTML XML Download Download as PDF (Size:660KB) PP. 512-520
DOI: 10.4236/aim.2016.67051    1,331 Downloads   1,908 Views Citations

ABSTRACT

Untreated Hospital wastewater piped into septic tanks contributes to the spread of antibiotic resistance in developing countries. This study was conducted to determine the resistant profile, and Extended Spectrum Beta-Lactamases (ESBLs) production in isolates from hospital waste water, of 2 hospitals in Delta State, Nigeria. A total of 147 organisms were isolated from 32 waste water samples. One hundred and twenty three isolates were Gram negative and 24 were Gram positive. Escherichia coli was the most prevalent in the two locations. Antimicrobial susceptibility by standard disk diffusion method was performed. All isolates were resistant to 4 or more antimicrobial agents. Out of the 123 Gram negative Bacteria, 33 were pan drug resistant and were selected for plasmid curing, DNA extraction and phenotypic detection of ESBL. Transfer of resistant by broth mating technique was performed. Plasmid curing and extraction result indicated that isolates carried resistance on the plasmid and harboured similar multiple high molecular weight plasmids of 23.13 kb and 9.4 kb. ESBL production was detected in 15.15%. Transfer of resistant genes between ESBL producing and non-ESBL producing isolates was observed. Incidence of ESBL in untreated hospital waste water has public health implications. Therefore establishment of treatment plants in our hospital is paramount in achieving sustainable health.

Cite this paper

Egbule, O. (2016) Detection and Transfer of Extended Spectrum Beta Lactamase Enzymes from Untreated Hospital Waste Water. Advances in Microbiology, 6, 512-520. doi: 10.4236/aim.2016.67051.

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.