Interspecific Crossing between Yam Species (Dioscorea rotundata and Dioscorea bulbifera) through in Vitro Ovule Culture - American Journal of Plant Sciences

AJPS > Vol.7 No.8, June 2016

Interspecific Crossing between Yam Species (Dioscorea rotundata and Dioscorea bulbifera) through in Vitro Ovule Culture ()

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Download as PDF (Size: 920KB) PP. 1268-1274

DOI: 10.4236/ajps.2016.78122 2,082 Downloads 3,281 Views Citations

Author(s)

Himanshu Saini, Yukiko Kashihara, Antonio Lopez-Montes, Robert Asiedu

Affiliation(s)

Genetic Resource Center, International Institute of Tropical Agriculture, HQ & West Africa Hub, Ibadan, Nigeria.

ABSTRACT

In the present study, in vitro ovule culture technique was used to obtain interspecific cross combination of Dioscorea rotundata ufenyi and Dioscorea bulbifera wild. Ten days after pollination, ovules were excised and cultured onto 1/2 strength Murashige and Skoog (MS) medium (Basal salt mixture + Vitamins) supplemented with 6% sucrose, 0.7% agar and plant growth hormones such as GA3, BAP, Picrolam and TDZ. Cultured ovules were transferred on 1/2 MS medium with 3% sucrose and 0.7% agar after three weeks. 40 days after pollination, germination was observed from 7 months cultured ovule between D. rotundata ufenyi x D. bulbifera wild. Hybridity of the regenerated plant was checked by flow cytometric method. A close relation was observed between the fluorescence intensity of the obtained progeny with one of the parents’ fluorescence. The observed progeny can be closely correlated with an apomictic tissue from an ovule parent of D. rotundata ufenyi. Plantlets derived from ovule culture were proliferated through in vitro shoot multiplication with hormonal concentration (0.5 mg/l BAP) supplemented with 1/2 strength MS medium. Obtained ovule culture derived in vitro plantlets were successfully hardened, acclimatized and transferred to the field, where they survived and grew normally. In plant breeding, interspecific crossing is very important technique, enabling the time needed to produce homozygous lines to be shortened as compared to the conventional plant breeding techniques.

KEYWORDS

Interspecific Crossing, Ovule Culture, in Vitro, Dioscorea rotundata, Dioscorea bulbifera

Share and Cite:

Saini, H. , Kashihara, Y. , Lopez-Montes, A. and Asiedu, R. (2016) Interspecific Crossing between Yam Species (Dioscorea rotundata and Dioscorea bulbifera) through in Vitro Ovule Culture. American Journal of Plant Sciences, 7, 1268-1274. doi: 10.4236/ajps.2016.78122.

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