Author(s)

Masakuni Tako^1,2*, Rintaro Ohtoshi¹, Kazutaka Kinjyo¹, Shuntoku Uechi¹

¹Department of Subtropical Bioscience and Biotechnology, University of the Ryukyus, Okinawa, Japan.
²Health and Longevity Research Laboratory, Integrated Innovation Research Center, University of the Ryukyus, Okinawa, Japan.

The polysaccharide was isolated from Hypnea pannosa which was grown in Okinawa, Japan. The yield of the polysaccharide was 17.2%, and the total carbohydrates, pyruvic acid, sulfuric acid and ash contents were 55.2%, 3.8%, 35.2% and 24.3%, respectively. 3,6-Anhydro-α-D-galactose, β-D-galactose, α-D-galactose and D-glucose were identified by liquid and thin-layer chromatography. Fourier transform infrared (FTIR) spectra of the polysaccharide resembled that of ι-carrageenan. From the ¹H- and ¹³C-NMR spectra, 1,3-linked β-D-galactose, 1,4-linked anhydro-α-D-galactose, 1,4-linked α-D-galactose, 1,4-linked β-D-glucose and pyruvic acid (carboxyl acetal, methyl proton and methyl carbon) were assigned. Methylation analysis revealed terminal D-galactose 0.1 mol), 1,4-linked D-glucose (1.0 mol) and 1,2,3,4,6-linked D-galactose (3.7 mol) for native polysaccharide, and terminal D-galactose, 1,4-linked D-galactose (1.9 mol), 1,4-linked D-glucose (1.0 mol), 1,3- linked D-galactose (1.7 mol), and 1,3,4,6-linked D-galactose (0.3 mol) which substituted with pyruvate group at 4 and 6 positions for desulfated polysaccharide. The polysaccharide was the novel pyruvated glucogalactan sulfate, the structure of which was proposed.

Hypnea pannosa, Pyruvated Glucogalactan Sulfate, ¹H- and ¹³C-NMR Analy-sis, Methylation Analysis, Chemical Structure

Tako, M. , Ohtoshi, R. , Kinjyo, K. and Uechi, S. (2016) The Novel Pyruvated Glucogalactan Sulfate Isolated from the Red Seaweed, Hypnea pannosa. Advances in Biological Chemistry, 6, 114-125. doi: 10.4236/abc.2016.63010.