Genetic Diversity of Tunisian and Chinese Alfalfa (Medicago sativa L.) Revealed by RAPD and ISSR Markers - American Journal of Plant Sciences

AJPS > Vol.7 No.6, April 2016

Genetic Diversity of Tunisian and Chinese Alfalfa (Medicago sativa L.) Revealed by RAPD and ISSR Markers ()

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DOI: 10.4236/ajps.2016.76092 2,311 Downloads 3,974 Views Citations

Author(s)

Leila Touil^1,2*, Aike Bao², Suomin Wang², Ali Ferchichi¹

Affiliation(s)

¹Arid Land and Oasis Cropping Laboratory, Institute of Arid Land, Medenine, Tunisia.
²State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.

ABSTRACT

Medicago sativa L.) is one of the most important forage crops in the world. The genetic variability analysis of 19 alfalfa populations collected from three sites in South Tunisia (Gabes, Kebili, Tozeur) and 1 from North West China were carried out using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markers. Five RAPD primers amplified 44 bands of which 22 were polymorphic; and five ISSR primers amplified 51 bands of which 33 were polymorphic. The percentage of polymorphic bands detected by RAPD and ISSR was 50% and 64.7%, respectively. The resolving power (Rp) varied between 0.6 and 4.1 with an average of 2.02 for RAPD marker and between 0.7 and 6.5 with an average of 2.28 for ISSR marker. However the Average Informativeness band (AvIb) was ranged from 0.2 to 0.9 with an average of 0.5 in RAPD marker and from 0.29 to 0.7 with an average of 0.624 in ISSR marker. The RAPD marker revealed less within population genetic diversity than ISSR marker. Although Cluster (UPGMA) and Correspondence Factorial Analyses (CFA) indicate that populations’ clustering made independently both from the geographical origin.

KEYWORDS

Medicago sativa L., Genetic Variability, RAPD, ISSR, UPGMA Clustering

Share and Cite:

Touil, L. , Bao, A. , Wang, S. and Ferchichi, A. (2016) Genetic Diversity of Tunisian and Chinese Alfalfa (Medicago sativa L.) Revealed by RAPD and ISSR Markers. American Journal of Plant Sciences, 7, 967-979. doi: 10.4236/ajps.2016.76092.

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