Author(s)

Éverson Miguel Bianco^1,2*, Simone Quintana de Oliveira^2*, Tatiana da Rosa Guimarães^2*, Luiza Gabriela de Souza Pessoa², Maria Eduarda Alves Santos³, Felipe Dal-Pizzol⁴, Eloir Paulo Schenkel², Flávio Henrique Reginatto²

¹Departamento de Ciências do Mar, Universidade Federal de Sao Paulo, Campus Baixada Santista, Santos, Brazil.
²Programa de Pós-Graduacao em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
³Molecular Invertebrate and Systematics Ecology Laboratory, Department of Biology, Chemistry and Marine Sciences, University of the Ryukyus, Nishihara, Japan.
⁴Departamento de Clínica Médica, Universidade Federal de Santa Catarina, Campus Universitário Trindade, Florianópolis, Brazil.

Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and other are an incredible source of novel pharmacologically active compounds. Herein, the antimicrobial activity (extract/fractions) of the invasive stony coralTubastraea coccinea was evaluated by the disk diffusion method against 21 microbial strains (ATCC and clinical strains). Micro broth dilution was used to determinate the MIC of the fractions that showed antimicrobial activity by the disk diffusion method. Bioautography assay was also performed. Our results showed that the n-butanol (BF) and aqueous fractions (AF) showed activity against ATCC strains Staphylococcus aureus (MIC 31.25 and 250 μg/mL),Salmonella typhimurium (MIC 125 and 500 μg/mL), Escherichia coli (MIC 62.5 and 500 μg/mL) and Pseudomonas aeruginosa (MIC 62.5 and 500 μg/mL), respectively. Moreover, BF fraction was also effective against the clinical strains S. aureus(MIC 62.5 μg/mL), Klebsiella pneumoniae Carbapenemase—KPC (MIC 125 μg/mL), Methicillin-Resistant Staphylococcus aureus—MRSA (MIC 125 μg/mL) and Vancomycin-Resistant Enterococcus faecalis—VRE (MIC 62.5 μg/mL). The ratio MBC/MIC reinforces the bactericidal profile of BF fraction. The bioautography assay of BF fraction showed the presence of antimicrobial components at R_f0.55.

Marine Natural Products, Tubastraea coccinea, Scleractinia, Antimicrobial Activity, Methicillin- Resistant Staphylococcus aureus (MRSA), Vancomycin-Resistant Enterococcus faecalis (VRE)

Bianco, É. , de Oliveira, S. , Guimarães, T. , de Souza Pessoa, L. , Santos, M. , Dal-Pizzol, F. , Schenkel, E. and Reginatto, F. (2016) Tubastraea coccinea: A Non-Indigenous Coral (Cnidaria, Scleractinia) Collected at Arvoredo Island, South of Brazil with Potential MRSA and VRE Antimicrobial Activity. Open Journal of Marine Science, 6, 334-340. doi: 10.4236/ojms.2016.62028.