Differential Expression of miRNAs in Sorghum bicolor under Drought and Salt Stress - American Journal of Plant Sciences

AJPS > Vol.7 No.6, April 2016

Differential Expression of miRNAs in Sorghum bicolor under Drought and Salt Stress ()

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DOI: 10.4236/ajps.2016.76082 2,409 Downloads 3,742 Views Citations

Author(s)

Rania S. El Sanousi¹, Nada B. Hamza^2*, Awadalla A. Abdelmula¹, Ismail A. Mohammed¹, Seif M. Gasim¹, Neeti Sanan-Mishra³

Affiliation(s)

¹Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan.
²Commission for Biotechnology & Genetic Engineering National Centre for Research, Khartoum, Sudan.
³Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, ArunaAsaf Ali Marg, New Delhi, India.

ABSTRACT

The regulatory mechanisms of drought and salt-associated miRNAs have not been fully understood in Sorghum bicolor. In this study, we investigated the effect of salinity stress (200 and 300 mM NaCl) and drought stress at pre- and post-flowering stages on the expression pattern of small regulatory RNAs in six Sorghum genotypes using semi-quantitative reverse transcriptase PCR (RT-qPCR). The results indicated that both drought and salt stresses altered the expression pattern of miRNAs in a dose-dependent manner. However, each miRNA responded to drought and salt stress in a different pattern among the six sorghum genotypes. miR156, miR167, miR168 and miR399 give different expressions levels compared to other studied miRNAs which may attribute to the adaption of sorghum to drought and salt stress and are good candidates for improving sorghum by transgenic technology.

KEYWORDS

miRNA, Up-Regulation, Down-Regulation, Pre-Flowering, Post-Flowering

Share and Cite:

El Sanousi, R. , Hamza, N. , Abdelmula, A. , Mohammed, I. , Gasim, S. and Sanan-Mishra, N. (2016) Differential Expression of miRNAs in Sorghum bicolor under Drought and Salt Stress. American Journal of Plant Sciences, 7, 870-878. doi: 10.4236/ajps.2016.76082.

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