A risk situation of organic pollutants accumulation in the sediments of the dams from Cascade “Middle Iskar” (NW Bulgaria) is simulated in the present study. The aim is risk modelling of organic pollution in lake sediments and on base of the investigated key chemical and enzymological parameters to risk assessment. A model process in lab scale with addition of whey as a source of organic pollutants was simulated. Two situations near to real conditions were studied: 1) at a decreased concentration of organic pollutants (chemical oxygen demand was 0.265 gO₂.kg^-1) in the case of discharge of sediments downstream of the river (control situation); 2) at an accumulation of organic pollutants (chemical oxygen demand was 1.463 gO₂.kg^-1) in the sediments (risk event). The dynamics of chemical oxygen demand (COD), concentration of proteins and lactose as well as of the key enzymological indicators (phosphatase activity index—PAI, anaerobic dehydrogenase activity, protease and ?-galactosidase activities) were investigated. The data from Risk Assessment showed that there is a hazard from higher protein concentration at the end of the process in comparison with the control situation. The risk about COD and lactose concentration during the risk modelling was acceptable. The obtained results showed that the PAI can be used as an indicator for early warning of the organic pollution with protein nature.