Author(s)

Peng Sun¹, Feng Yan², Jinning Song^1*, Xudong Ma¹

¹Department of Neurosurgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China.
²Department of Nutrition, The Xijing Hospital, Fourth Military Medical University, Xi’an, China.

Objective: To investigate the role of adiponectin in human glioma cell lines against the temozolomide and the molecular regulation mechanism. Methods: Human glioma cell lines U251 and U-87MG were cultured in Dulbecco’s modified eagle medium (DMEM) containing 4500 mg/L glucose. MTT was used to measure cell growth ratio. Western blot was used to detect the protein levels of autophagy-related protein (Beclin 1, LC3 I/II, p62) and phosphorylated AMPK (p-AMPK) in human glioma cell lines. After AICAR and Compound C were administered, the change of p-AMPK and the autophagy level were examined by western blot. Results: While adiponectin stimulates AMPK in phosphatase and up-regulates the level of autophagy, human glioma cell lines obtain more resistance against the temozolomide, which is facilitated by AICAR and weakened by Compound C. Conclusion: As an important adipokine, adiponectin can up-regulate the glioma cell autophagy by activating the AMPK signaling pathway which increases the resistance of glioma cells to temozolomide.

Human Glioma Cell Lines, Adiponectin, Autophagy, Temozolomide, AMPK

Sun, P. , Yan, F. , Song, J. and Ma, X. (2016) Adiponectin Supports Human Glioma Cells Survival against Temozolomide through Enhancement of Autophagic Response in Glioma Cells. Journal of Biosciences and Medicines, 4, 1-9. doi: 10.4236/jbm.2016.44001.