Author(s)

Hao Qian, Danyi Lu, Wan Li, Xiaotong Zhou, Baojian Wu, Zhiguo Ma^*

Division of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou, China.

Poliumoside is the main active constituent of Callicarpa kwangtungensis Chun (CK), a traditional Chinese medicine for management of hemostasis. In this study, a rapid and selective ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/ Q-TOF-MS) method was developed and validated to quantify poliumoside in rat plasma. The targeted analytes in rat plasma were prepared through protein-precipitation method using 10% trichloroacetic acid (TCA). The chromatographic separation was performed on a Waters BEH C₁₈ column (2.1 × 100 mm, 1.7 μm) by acetonitrile-water containing 0.1% formic acid. The calibration curve was linear over the range of 50 - 10,000 ng/mL (r² > 0.99). The intra-day or inter-day precision was less than 7.97% and accuracy was within ?7.00% - 3.36%. The developed method was successfully applied to pharmacokinetic study of poliumoside in rat plasma. Although being rapidly absorbed (T_max ≤ 30 min), poliumoside was poorly bioavailable after oral administration (the absolute bioavailability was only 0.69%).

Poliumoside, UPLC/Q-TOF-MS, Rat Plasma, Pharmacokinetics

Qian, H. , Lu, D. , Li, W. , Zhou, X. , Wu, B. and Ma, Z. (2016) Validated UPLC/Q-TOF-MS Method for Determination of Poliumoside in Rat Plasma and Its Application to Pharmacokinetic Study. American Journal of Analytical Chemistry, 7, 266-274. doi: 10.4236/ajac.2016.73024.