Author(s)

Charlemagne Ouédraogo^1,2*, Théodora Mahoukèdè Zohoncon^1,3,4,5, Esther M. A. Traoré³, Souleymane Ouattara⁴, Prosper Bado³, Clarisse T. Ouedraogo³, Florencia W. Djigma^1,3, Djénéba Ouermi^1,3, Dorcas Obiri-Yeboah⁶, Olga Lompo^4,7, Simon A. Akpona⁶, Jacques Simpore^1,3,5

¹Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso.
²UFR/SDS, University of Ouagadougou, Ouagadougou, Burkina Faso.
³Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, Ouagadougo, Burkina Faso.
⁴Teaching and Research Unit in Biochemistry and Molecular Biology, Faculty of Medicine, University of Parakou, Parakou, Republic of Benin.
⁵Faculty of Medicine, Université Saint Thomas d’Aquin, Ouagadougou, Burkina Faso.
⁶University of Cape Coast, School of Medical Sciences, Microbiology Department, University Post Office, Cape Coast, Ghana.
⁷Yalgado Ouédraogo University Hospital (CHU/YO), Ouagadougou, Burkina Faso.

Aims: We aimed at identifying the high-risk HPV genotypes associated with high-grade dysplastic cervical lesions in Burkina Faso. The available vaccines to Burkina Faso only protect against two high risk HPV genotypes: HPV 16 and 18. Are the genotypes identified in the high-grade precan-cerous lesions in this survey covered by the available vaccines? Methods: The detection and genotyping of high-risk HPV have been conducted based on 118 formalin-fixed and paraffin-embedded archived tissues using the “HPV Genotypes 14 Real-TM Quant” (Sacace biotechnologies^®, Italy) kit allowing for the detection of fourteen high-risk HPV genotypes: HPV 16, 31, 18, 39, 45, 59, 33, 35, 56, 68, 51, 52, 58 and 66. Results: The prevalence of high-risk HPV infections was 48.8% based on the appropriate PCR results (21/43). The most common HPV genotypes were HPV 39 (21.7%), HPV 35 (13.0%) and HPV 45 (13.0%). Two cases of multiple infections between HPV 39 - 45 and HPV 39 - 59 have been observed. HPV 16 was not detected in this study. Conclusions: We noted a high prevalence rate for HPV 39, HPV 35 and HPV 45, which are not covered by the commercial vaccines. We also found that the prevalence of HPV 18 was very low in this study and HPV 16 was not detected.

HPV, Cancer, CIN, Cervix, Burkina Faso

Ouédraogo, C. , Zohoncon, T. , Traoré, E. , Ouattara, S. , Bado, P. , Ouedraogo, C. , Djigma, F. , Ouermi, D. , Obiri-Yeboah, D. , Lompo, O. , Akpona, S. and Simpore, J. (2016) Distribution of High-Risk Human Papillomavirus Genotypes in Precancerous Cervical Lesions in Ouagadougou, Burkina Faso. Open Journal of Obstetrics and Gynecology, 6, 196-204. doi: 10.4236/ojog.2016.64025.