Main pollutants of the urban scenic river in Nanjing City are studied in this paper. In the study area a total of 39 monitoring points are set in natural water, around pumping stations and near the tail water of sewage treatment plant. Through the monitoring of pollution sources of receiving conventional index, the pollution sources distribution and river pollution factors are detailed analyzed, as nutrient salts, heavy metals, and environmental endocrine disruptors. And sources of the pollution factors are analyzed by principal component analysis to get the main pollution factors in this channel.