Author(s)

Ahou Anique Gbotto^1*, Kouamé Kevin Koffi¹, Jean-Pierre Baudoin², Irié Arsène Zoro Bi¹

¹Laboratoire de Génomique Fonctionnelle et Amélioration Génétique (LaGeFAGe), UFR-SN, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire.
²Unité de Phytotechnie Tropicale et d’Horticulture, Gembloux Agro Bio Tech, Université de Liège, Gembloux, Belgium.

Thirty accessions of Lagenaria siceraria from the Nangui Abrogoua University germplasm collection were analyzed using three microsatellite markers. The average Polymorphism Information Content (PIC) value was 0.61. The average observed heterozygosity value (H0 = 0.631) compared to the average expected heterozygosity value (He = 0.645) did not show significant differences in the selected accessions, which suggested random mating in the set of accessions. Within accession inbreeding estimate (FIS), was 40% and was not significantly different from zero. The reduction of heterozygotes was likely the result of presence of null alleles. Analysis of Molecular Variance (AMOVA) within and among 30 accessions of L. siceraria revealed that 39% of the total variation resides among accessions and 61% within accessions. Accession structuring pattern derived from Bayesian clustering analysis revealed two clusters. Based on the genetic structure of the accessions analyzed, a sampling strategy to collect and conserve genetic resources of L. siceraria was suggested.

Genetic Structure, Lagenaria siceraria, Null Alleles, Oleaginous, SSR

Gbotto, A. , Koffi, K. , Baudoin, J. and Zoro Bi, I. (2015) Determination of the Genetic Structure of the Oleaginous Lagenaria siceraria of the Nangui Abrogoua University Germplasm Collection. American Journal of Plant Sciences, 6, 3231-3243. doi: 10.4236/ajps.2015.619314.