ABSTRACT
The antioxidant and protective properties of a
synthetic soybean isoflavone (ISO) were investigated using sow mammary gland
cell. Cells were cultured with 10, 20 and 30 uM ISO, respectively, under 80 uM
FeSO4·7H2O/H2O2 conditions. After 48 h of incubation, the cells in the presence of ISO were
lost less compared with that of control under oxidative damage by H2O2/FeSO4;
ISO decreased the cell number at G1 and G2 stages, increased the cell number at
S stage (all P < 0.05), it also reduced
apoptosis of the cells (P < 0.01, P < 0.01, P < 0.05). The addition of ISO significantly promoted cell
proliferation (P < 0.05) from 3rd to 6th days. Upon these, the activities of total superoxide
dismutase (SOD), glutathione peroxidase (GPX), total antioxidant ability (T-AOC)
also were increased and the activities of NADPH oxidase (NOX) decreased by ISO
treatment (all P < 0.05). ISO
decreased the concentration of ROS (P < 0.05) and MDA (P < 0.05). 10
uM, 20 uM, 30 uM ISO increased the relative mRNA abundance of SOD1 (all P < 0.05) and SOD2 (P < 0.05, P < 0.01, P < 0.05)
and SOD3 (all P < 0.05); ISO
significantly increased the relative mRNA abundance of GPX4 (all P < 0.01) and NOX4 (all P < 0.05), 20 uM ISO also increased
the relative mRNA abundance of NOX2 (P <
0.05). It was concluded that supplementation of ISO enhanced the anti-oxidative
function and prevented lipid peroxidation, possibly
through the activation of the antioxidant enzymes and inhibition of cell
apoptosis.
Share and Cite:
Ma, X. , Jiang, Z. , Zhang, J. , Hu, Y. , Gao, K. , Wang, L. and Yang, X. (2015) Isoflavone Ameliorates H
2O
2 Induced Injury by Activating the Antioxidant System of Sow Mammary Gland Cell.
Natural Science,
7, 571-580. doi:
10.4236/ns.2015.712057.