Author(s)

Zuraida Ab Rahman¹, Erny Sabrina Mohd Noor², Mohd Shukri Mat Ali², Razali Mirad², Ayu Nazreena Othman¹

¹Biotechnology Research Centre, MARDI Headquarters, MARDI HQ, Persiaran MARDI-UPM, Serdang, Malaysia.
²Strategic Resource Centre, MARDI HQ, Persiaran MARDI-UPM, Serdang, Malaysia.

The effect of the plant growth regulators benzyl amino purine (BAP), 1-naphthaleneacetic acid (NAA) and kinetin (KIN) on in vitro shoot induction and proliferation of Plectranthus amboinicus was examined. Explants obtained from lateral shoots and apical shoots of P. amboinicus were inoculated on Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP, NAA and KIN. When the effect of each growth regulator was considered singly, the highest rate of shoot induction (80% of explants producing shoots) and highest number of shoots produced (2.4 shoots per explant) were obtained from lateral shoot explants cultured on MS media supplemented with 3.0 mg/L BAP within 6 - 7 weeks. Better results were obtained using MS medium supplemented with 1 mg/L BAP + 5 mg/L NAA. Shoot proliferation rose to 85%, while 5.7 shoots per explants were recorded. Among the different media tested for rooting, MS medium supplemented with 1.0 mg/L IBA was the most effective for root induction. The quality of the roots obtained was better than that obtained using MS media supplemented with NAA or IAA.

Plectranthus amboinicus, Micropropagation, Medicinal Plant, Plant Growth Regulator

Rahman, Z. , Mohd Noor, E. , Mat Ali, M. , Mirad, R. and Othman, A. (2015) In Vitro Micropropagation of a Valuable Medicinal Plant, Plectranthus amboinicus. American Journal of Plant Sciences, 6, 1091-1097. doi: 10.4236/ajps.2015.68113.