Author(s)

Chi-You Wu¹, Chih-Ping Hsu², Chih-Cheng Lin³, Fung-Jou Lu⁴, Chiu-Chen Huang⁵, Yi-Hsien Lin², Ching-Hsein Chen^6*

¹Graduate Institute of Food Science and Biopharmaceutics, National Chiayi University, Chiayi City.
²Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu City.
³Department of Biotechnology, Yuanpei University, Hsinchu City.
⁴Institute of Medicine, Chung Shan Medical University, Taichung City.
⁵Department of Anesthesiology, China Medical University Hospital, Taichung City.
⁶Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi City.

Flesh extract of Mangifera indica possesses anti-proliferation effect on different types of cancer cells. However, the effect of its seed kernel extract (MSP) on the growth of human colorectal carcinoma cells (CRC) has not yet been evaluated. Phenolic species of MSP were extracted and measured by colorimetry. Two CRC cell lines (Colo 320DM and SW480) were treated with MSP and assessed for viability by trypan blue exclusion, for cell cycle distribution by flow cytometry, for apoptosis by annexin V labeling, for mitochondria potential by rhodamine 123 staining and for changes in the levels of proteins involved in cell cycle control or apoptosis by immunoblotting. MSP inhibited the proliferation (12.5 μg/mL - 50 μg/mL) of Colo 320DM and SW480. MSP inhibited proliferation by blocking cell cycle progression at G1 (SW480) or S (Colo 320DM) phase and inducing apoptotic death. Western blotting indicated that MSP-blocking cell cycle was associated with cyclin levels. MSP-treated Colo 320DM and SW480 also showed activation of caspase 8, 9 and 3. MSP induces cell cycle arrest and apoptotic death in two CRC cell lines. The results indicate that MSP is a potential novel chemoprevention and treatment agent for colorectal cancer.

Mangoseed Kernel Extract, Colorectal Carcinoma, Cell Cycle, Apoptosis

Wu, C. , Hsu, C. , Lin, C. , Lu, F. , Huang, C. , Lin, Y. and Chen, C. (2015) Different Mechanisms of Seed Kernel Extract from Mangifera indica on the Growth of Two Colon Cancer Cell Lines. Food and Nutrition Sciences, 6, 421-428. doi: 10.4236/fns.2015.64043.