Author(s)

Qi Wang¹, Bihua Duan^2*, Bihua Duan², Rui Yang², Yaorong Zhao¹, Lu Zhang¹

¹College of Landscape Architecture, Beijing University of Agriculture, Beijing, China.
²College of Plant Science and Technology, Beijing University of Agriculture/Beijing Key Laboratory for Agricultural Application and New Technique, Beijing, China.

Objective: This study aimed to separate and pure chitinase producing actinomycetes with stronger activity from lawn soils of Beijing. Method: Morphology and culture characteristics were observed. physiological and biochemical characteristics were detected. 16SrDNA sequence was analyzed. Result: The highest chitinase activity strain was Bn035, whose relative diameter of transparent circle K2 was 3.42 at which the chitinase activity of fermentation broth reached a maximum value of 1.23 U/mL in the 6th day. Crude enzyme solution and bacteria cake of strain Bn035 generated stronger bacterial resistance to all root rot fungi which indicated that the strain had a strong broad-spectrum resistance to root rot fungi. Conclusion: The strain was identified as Streptomyces scabrisporus. It will be a potential biological control function.

Strain Bn035, Transparent Circle, Chitinase Activity, Antagonism, 16SrDNA

Wang, Q. , Duan, B. , Duan, B. , Yang, R. , Zhao, Y. and Zhang, L. (2015) Screening and Identification of Chitinolytic Actinomycetes and Study on the Inhibitory Activity against Turfgrass Root Rot Disease Fungi. Journal of Biosciences and Medicines, 3, 56-65. doi: 10.4236/jbm.2015.33009.