Soil carbon content is an important ecosystem property, especially under the ongoing climate change. The stability of soil organic matter (SOM) is controlled by environmental and biological factors including anthropogenic-induced agricultural management change. However, understanding the effects of anthropogenic activities (e.g., intensive agricultural practices) on carbon stability of soil profiles remains a challenge. The objective of this study was to determine the changes in carbon stocks through soil profiles following agricultural management change from grain fields to greenhouse vegetable fields. The sampling sites were located in an intensive vegetable production area in northernChina. A total of 20 pairs of grain fields (GF) and adjacent vegetable fields (VF) within a distance of50 mwere selected. The results showed that soil organic carbon (SOC) storage increased by 10.6 mg C ha^-1 in upper soil layers but decreased by 5.3 mg C hm² indeeper soil layers due to large input of organic manure and chemical fertilizer following the conversion from GF to VF. Conversion to VF also led to increased dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) concentrations. Extremely higher input of chemical N fertilizer in the VF led to the soil C:N ratio decreased by 2.02 times and the -N leached to deeper soils increased by 3.7 times compared to that in the GF. The pH value and microbial biomass carbon (MBC) content were lower in the VF than in the GF. These results indicate that excessive nitrogen application as fertilizers might lead to deeper soil carbon depletion. Reducing nitrogen addition in intensive agricultural systems is thus necessary to reduce soil carbon loss and to maintain a relatively sustainable soil system.