Author(s)

Reginald S. Fletcher¹, James R. Smith², Alemu Mengistu³, Jeffery D. Ray²

¹Crop Production Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, USA.
²Crop Genetics Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, USA.
³Crop Genetics Research Unit, Agricultural Research Service, United States Department of Agriculture, Jackson, USA.

Alternative methods are needed to assess the severity of charcoal rot disease [Macrophomina phaseolina (Tassi) Goid] in soybean [Glycine max (L.)] plant tissue. The objective of this study was to define the relationship between light reflectance properties and microsclerotia content of soybean stem and root tissue. Understanding that relationship could lead to using spectral reflectance data as a tool to assess the severity of charcoal rot disease in soybean plants, thus reducing human bias associated with qualitative analysis of soybean plant tissue and cost and time issues connected with quantitative analysis. Hyperspectral reflectance measurements (400-2490 nm) were obtained with a non-imaging spectroradiometer of non-diseased and charcoal rot diseased ground stem and root tissue samples of six soybean genotypes (“Clark”, “LD00-3309”, “LG03- 4561-14”, “LG03-4561-19”, “Saline”, and “Y227-1”). Relationships between the reflectance measurements and tissue microsclerotia content were evaluated with Spearman correlation (rs) analysis (p < 0.05). Moderate (rs = ±0.40 to ±0.59), strong (rs = ±0.60 to ±0.79), and very strong (rs = ±0.80 to ±1.00) negative and positive statistically significant (p < 0.05) monotonic relationships were observed between tissue spectral reflectance values and tissue microsclerotia content. Near-infrared and shortwave-infrared wavelengths had the best relationships with microsclerotia content in the ground tissue samples, with consistent results obtained with near-infrared wavelengths in that decreases in near-infrared spectral reflectance values were associated with increases in microsclerotia content in the stem and root tissue of the soybean plants. The findings of this study provided evidence that relationships exist between tissue spectral reflectance and tissue microsclerotia content of soybean plants, supporting spectral reflectance data as a means for assessing variation of microsclerotia content in soybean plants. Future research should focus on the modelling capabilities of the selected wavelengths and on the feasibility of using these wavelengths in machine learning algorithms to differentiate non-diseased from charcoal rot diseased tissue.

Hyperspectral, Stem-Root Tissue, Charcoal Rot, Macrophomina phaseolina, Soybeans

Fletcher, R. , Smith, J. , Mengistu, A. and Ray, J. (2014) Relationships between Microsclerotia Content and Hyperspectral Reflectance Data in Soybean Tissue Infected by Macrophomina phaseolina. American Journal of Plant Sciences, 5, 3737-3744. doi: 10.4236/ajps.2014.525390.