Author(s)

Susheela Rani, Ashwini Kumar, Ashok Kumar Malik, P. Schmitt-Kopplin

.

This study presents a fast, accurate and sensitive technique using gas chromatography-mass spectrometry (GC-MS) for the identification and quantification of N-acyl homoserine lactones (AHLs) in the extracts of bacterial strain of Pseudomonas aeruginosa and sputum sample of a cystic fibrosis patient. This method involves direct separation and determination of AHLs by using GC-MS as simultaneous separation and characterization of AHLs were possible without any prior derivatiza-tion. Electron ionization resulted in a common fragmentation pattern with the most common fragment ion at m/z 143 and other minor peaks at 73, 57 and 43. The limit of detection for N-butanoyl, N-hexanoyl, N-octanoyl, N-decanoyl, N-dodecanoyl and N-tetradecanoyl homoserine lactones was 2.14, 3.59, 2.71, 2.10, 2.45 and 2.34 µg/L, respectively. The presence of AHLs in the culture of P. aeruginosa strain and spu-tum of a cystic fibrosis patient was achieved in selected ion monitoring (SIM) mode by using the prominent fragment at m/z 143.

Gas Chromatography—Mass Spectrometry, N-Acyl Homoserine Lactone (N-Butanoyl, N-Hexanoyl, N-Octanoyl, N-Decanoyl, N-Dodecanoyl and N-Tetradecanoyl) Homoserine Lactone, Sputum Sample, Bacterial Strain

S. Rani, A. Kumar, A. Malik and P. Schmitt-Kopplin, "Occurrence of N-Acyl Homoserine Lactones in Extracts of Bacterial Strain of Pseudomonas aeruginosa and in Sputum Sample Evaluated by Gas Chromatography–Mass Spectrometry," American Journal of Analytical Chemistry, Vol. 2 No. 2, 2011, pp. 294-302. doi: 10.4236/ajac.2011.22037.