Author(s)

Saroj Kumar Sah^*, Gurwinder Kaur, Amandeep Kaur

School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India.

The isolation of high quality RNA is a crucial technique in plant molecular biology. The quality of RNA determines the reliability of downstream process like real time PCR. In this paper, we reported a high quality RNA extraction protocol for a variety of plant species. Our protocol is time effective than traditional RNA extraction methods. The method takes only an hour to complete the procedure. Spectral measurement and electrophoresis were used to demonstrate RNA quality and quantity. The extracted RNA was further used for cDNA synthesis, expression analysis and copy number determination through Real Time PCR. The results indicate that RNA was of good quality and fit for real time PCR. This high throughput plant RNA extraction protocol can be used to isolate high quality RNA from diverse plants for real time PCR and other downstream applications.

RNA Extraction, Diverse Plants, Trizol, High Quality Protocol, Real Time PCR

Sah, S. , Kaur, G. and Kaur, A. (2014) Rapid and Reliable Method of High-Quality RNA Extraction from Diverse Plants. American Journal of Plant Sciences, 5, 3129-3139. doi: 10.4236/ajps.2014.521329.