Author(s)

Liana Ramishvili¹, Irma Bochorishvili¹, Nino Gabunia², Zurab Kuchukashvili¹, Manana Gordeziani¹, Teimuraz Chigogidze², Ana Khazaradze¹, Zaza Melikishvili³, Nanuli Kotrikadze^1*

¹Department of Biology, Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia.
²Department of Urology, Faculty of Medicine, Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia.
³Institute of Cybernetics of the Academy of Sciences, Tbilisi, Georgia.

Objectives: Fluorescence spectroscopy which can be used for optical tissue diagnosis of tumor pathology deserves special interest. The purpose of the work was to study blood plasma and tumor tissue of men with different forms of prostate tumors by using laser induced fluorescence. Blood plasma and tumor tissue of the patients with benign hyperplasia of the prostate (BHP), BHP with inflammation, BHP with high grade PIN (BHP with HGPIN) and adenocarcinoma of prostate (CaP) have been studied. Results: In case of blood plasma fluorescence, intensity of the plasma proteins corresponding peak (340 - 360 nm) was increasing in the following manner: control group → BHP → BHP with HGPIN → CaP. The intensity of the nicotinamide coenzymes correspond peak (440 - 460 nm) was increased in case of BHP with HGPIN and CaP patients, but decreased in case of BHP, compared to control. In case of tumor tissue, the changes of the collagen peak (390 - 400 nm) intensity have been revealed in all cases of prostate tumor tissues. These alterations point to altered collagen biosynthesis levels in different tumor tissues, that reflects the structural changes and characteristics of malignant transformation. Also the changes of the nicotinamide coenzymes peak (440 - 460 nm) intensity in all spectra of tumor tissues were observed. The highest intensity of the peak was observed in the spectra of BHP with HGPIN and in prostate cancer tissue. Conclusions: Alterations of the coenzymes peak intensities perfectly reflect and are in accordance with the specific energy metabolism of prostate epithelial cells. Normalization of fluorescent spectra from different forms of prostate tumor tissues has shown that, each form has typical spectral shape and ratio of fluorescence peaks intensities.

Prostate Tumors, Fluorescence, Endogenous Fluorophores, Energy Metabolism

Ramishvili, L. , Bochorishvili, I. , Gabunia, N. , Kuchukashvili, Z. , Gordeziani, M. , Chigogidze, T. , Khazaradze, A. , Melikishvili, Z. and Kotrikadze, N. (2014) Laser Induced Fluorescence Studies of Blood Plasma and Tumor Tissue of Men with Prostate Tumors. Journal of Cancer Therapy, 5, 1021-1030. doi: 10.4236/jct.2014.511107.