Healthcare information systems are crucial components for better coordination of healthcare. They focus on the proper generation, transmission, storage, and retrieval of health data. It is obvious that production of accurate, relevant, and timely health information is foundation of good decision making. Rapid progress in wireless communications and embedded systems result in wireless sensor networks to be employed even in biomedical applications as well as their prominent deployment options. This study proposes a healthcare information system framework which consists of such components as; wireless sensor networks, cellular networks, a MATLAB interface, a database, and a web based monitoring interface. A case study that includes sensing, transferring, storing and web based monitoring processes of ECG signal is also introduced in the study, so that the behavior of the system developed can be tested. The results show that the framework presented here can not only be employed as a healthcare information system, but it can also be used as an infrastructure in related research activities and consequently, lots of time can be saved from creating an experimental environment.