The antifungal activity of ethanol and acetone extract of leaves of nine medicinal plants: Piper betel, Lowsonia inermis, Psidium guajava, Carica papaya, Moringa oleifera, Mimosa pudica, Catharanthus roseus, Adhatoda vasica and Andrographis paniculata against Fusarium oxysporum the causal agent of Fusarium wilt in tomato was assessed. All the extracts inhibited mycellial growth at various levels. Among them the superior inhibition (100%) was found in 15% concentration of ethanol extract of Lowsonia inermis and Psidium guajava against Fusarium. In all plant extract there were no significant differences between 20% and 25% concentration, except Piper betel, Carica papaya, Andrographis paniculata and Lawsonia inermis. Analysis of variance results on mycelial growth in different concentration shows that the item was highly significant.