We
determined vitamin B
12 content of Chinese black tea leaves using a
microbiological assay based on
Lactobacillus
delbrueckii ATCC 7830. Trace levels (0.25 - 0.69 μg/100g dry weight) of vitamin
B
12 were detected in Pu’er, Fu, and Brick tea leaves. However,
vitamin B
12 content (0.06 - 1.37 μg/100g dry weight) of Ryubao tea
leaves significantly varied. To determine whether Chinese black tea leaves
contain vitamin B
12 or other corrinoid compounds that are inactive
in humans, corrinoid compounds were purified from Ryubao tea by an immunoaffinity
column chromatography and vitamin B
12 was identified by liquid
chromatography-electrospray ionization/tandem mass spectrometry. Vitamin B
12 content in the tea drink prepared from Ryubao tea leaves was very low (0.8
ng/100mL). Our results indicate that Chinese black tea is usually not a good
source of B
12, although Ryubao tea leaves with the highest B
12 content may be utilized as a source of this vitamin for vegerarians.