Objective: To investigate college students’ implicit attitude of network altruistic behavior tendency. Methods: Choose 50 students from a university in Zhengzhou City who take psychology as an optional course to be the tested and conduct the IAT test on their implicit attitudes towards network altruistic behavior tendency. And D value, reaction time mean and Logarithm of three ways of numerical implicit effect check IAT experiment within the significant. Results: A significant IAT effect was found in implicit attitudes towards network altruistic behavior tendency; a significant difference appears in the students who major in art or science; no difference was found in gender. Conclusion: IAT is an effective tool can be used to measure if college students have the implicit attitudes towards network altruistic behavior tendency.