Author(s)

Nadèje Tekaya, Olga Saiapina, Hatem Ben Ouada, Florence Lagarde, Philippe Namour, Hafedh Ben Ouada, Nicole Jaffrezic-Renault

Institute of Analytical Sciences, University of Lyon, Villeurbanne, France.
Institute of Molecular Biology and Genetics of National, Academy of Sciences of Ukraine, Kyiv, Ukraine.
Laboratory of Interfaces and Advanced Materials, Faculty of Sciences, University of Monastir, Monastir, Tunisia.
National Institute of Marine Sciences and Technologies, University of Monastir, Monastir, Tunisia.

An original bi-enzymatic biosensor was designed by immobilizing Arthrospira platensis cells, called Spirulina, on gold interdigitated transducers. Phosphatase and esterase activities were inhibited, respectively, by heavy metals and by pesticides. Inhibition activities were observed with different mixtures of pesticides + heavy metals. The quantification limits for Cd²⁺ and Hg²⁺ are 10^-20 M in mixture and in pure solution. The quantification limits of parathion-methyl, paraoxon-methyl and triazine are respectively 10^-20 M, 10^-18 M and 10^-20 M in mixture and pure solutions. These results show that there is no synergistic effect between the two families of pollutants. Qualitative contamination of effluent samples and their purification after passing through a municipal wastewater treatment plant were observed by our bi-enzymatic biosensor and confirmed by classical analytical techniques for heavy metal ions.

Inhibition, Phosphatase Activity, Esterase Activity, Conductometry, Arthrospira platensis

Tekaya, N. , Saiapina, O. , Ouada, H. , Lagarde, F. , Namour, P. , Ouada, H. and Jaffrezic-Renault, N. (2014) Bi-Enzymatic Conductometric Biosensor for Detection of Heavy Metal Ions and Pesticides in Water Samples Based on Enzymatic Inhibition in Arthrospira platensis. Journal of Environmental Protection, 5, 441-453. doi: 10.4236/jep.2014.55047.