Author(s)

Yoshinari Taguchi, Ryohei Yamamoto, Natsukaze Saito, Masato Tanaka

Department of Chemistry and Chemical Engineering, Niigata University, Niigata, Japan.

Microcapsules containing the aqueous solution of Azur B of a water soluble dye were prepared with the melting dispersion cooling method and applied to the amplification detector of plant DNA. Paraffin wax with melting temperature of 75°C was used as the shell material. In the experiment, the aqueous solution (W) of Azur B as the core material was dispersed in the melted paraffin wax (O) to form the (W/O) emulsion and then, the (W/O) emulsion was dispersed in the silicon oil (O’) as the continuous phase to form the (W/O)/O’ emulsion at 85°C. After formation of the (W/O)/O’ emulsion, the microcapsules were prepared by cooling the (W/O)/O’ emulsion to 50°C. The microcapsules were prepared by changing the concentration of oil soluble surfactant in the (W/O) emulsion and the volume of the (W/O) emulsion in the (W/O)/O’ emulsion. The microencapsulation efficiency increased with the concentration of oil soluble surfactant and finally became 100% under the optimum conditions. Furthermore, the microcapsules were melted down at temperature of 85°C to reveal the sharp thermal responsibility and to release the aqueous solution of Azur B. As a result, it was found that the microcapsules were able to be applied to the amplification detector of plant DNA by utilizing the reaction between DNA and Azur B.

Microcapsules; Azur B; DNA Amplification Detector; Melting Dispersion Cooling Method; Multiple Emulsion

Taguchi, Y. , Yamamoto, R. , Saito, N. and Tanaka, M. (2014) Preparation of Microcapsules Containing Aqueous Solution of Azur B with Melting Dispersion Cooling Method and Application to DNA Amplification Detector. Journal of Encapsulation and Adsorption Sciences, 4, 15-24. doi: 10.4236/jeas.2014.41003.