Author(s)

Yuka Satoh, Hatsue Ogawara, Osamu Kawamura, Yasuyuki Shimoyama, Motoyasu Kusano, Akihiko Yokohama, Takayuki Saitoh, Hiroshi Handa, Norifumi Tsukamoto, Hirokazu Murakami

Department of Blood Transfusion Service, Gunma University Hospital, Maebashi, Japan.
Department of Endoscopy and Endoscopic Surgery, Gunma University Hospital, Maebashi, Japan.
Department of Laboratory Sciences, Course of Health Sciences, Gunma Graduate School of Health Sciences, Maebashi, Japan.
Department of Medicine and Clinical Science, Gunma Graduate School of Medicine, Maebashi, Japan.
Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi, Japan.
Faculty of Health Sciences, Gunma Paz College, Takasaki, Japan.
Oncology Center, Gunma University Hospital, Maebashi, Japan.

Helicobacter pylori (H. pylori) induces gastroduodenal diseases and vigorous humural and cellular immune abnormalities. In order to clarify the immunological changes before and after eradication of H. pylori, the percentages and ratios of the following cells in the peripheral blood of 32 H. pylori-infected patients and 25 control subjects were analyzed: CD4+ T cells, CD8+ T cells, T helper 1 cells (Th1), T helper 2 cells (Th2), CD4+CD25+ T cells, Foxp3+ regulatory T cells (Treg), CD4/CD8 ratio, and Th1/Th2 ratio. CD4/CD8 ratio was significantly higher in H. pylori-infected patients before (mean ± SD, 2.9 ± 1.9) and after (mean ± SD, 2.8 ± 1.6) eradication of H. pylori than in control subjects (mean ± SD, 2.1 ± 0.9). The percentage of Th2 cells was significantly higher in H. pylori-infected patients (mean ± SD, 2.6 ± 1.1) than in control subjects (mean ± SD, 1.9 ± 1.1; p < 0.02). The percentage of Th2 cells after eradication of H. pylori (mean ± SD, 2.3 ± 1.4) was lower than that before eradication. There was no significant difference between control subjects (mean ± SD, 4.1% ± 1.5%) and patients before H. pylori eradication (mean ± SD, 4.5% ± 2.4%) in the percentage of Tregs, but the percentage was significantly higher in patients after H. pylori eradication (mean ± SD, 5.2% ±2.6%) than in control subjects. The function of peripheral induced Tregs was reported to suppress the excessive immune reaction in chronic inflammation. These data suggest that Tregs may proliferate and be activated to suppress the activation of humoral immunity in H. pylori-infected patients, and these changes continue after 3 months or later of successful eradication of H. pylori.

Helicobacter pylori; Regulatory T Cell; Th1 Cell; Th2 Cell

Satoh, Y. , Ogawara, H. , Kawamura, O. , Shimoyama, Y. , Kusano, M. , Yokohama, A. , Saitoh, T. , Handa, H. , Tsukamoto, N. and Murakami, H. (2014) Regulatory T cells percentage in peripheral blood before and after eradication of Helicobacter pylori. Health, 6, 236-242. doi: 10.4236/health.2014.64035.