A system which consists of a loading chamber unit, displacement sensor, data collector and processor, and a feedback control, was established for applying mechanical forces to single plant cells. The method works by compressing an agar cell-suspension block between parallel surfaces through using a force-feedback control circuit coupled to a microchip, delivering the pre-defined. The actual controlled stimulus is achieved whilst measuring the force being imposed on the cell, and its deformation. TheArabidopsisprotoplasts were utilized to test the system. It provides an experimental approach to investigate the mechanoresponses of plant cellsin vitroconditions.