In the present work, Compound 1 has been synthesized as a novel fluorescence ratiometric probe for CN^-. Upon treatment with CN^-, Probe 1 exhibited a fluorescence ratiometric response, with the emission wavelength shift from 570 nm to 608 nm. When 90 μM CN^- was introduced, the emission ratios (I₅₇₀/I₆₀₈) of the probe changed dramatically from 0.52156 to 4.21472. The detection limit was also measured to be 0.24 μM (S/N = 3). In addition, Probe 1 had a selective response to CN^-, while other anions caused nearly no interference. The sensing reaction product of Probe 1 with CN^- was characterized by ¹H NMR spectra and ESI Mass spectrometry. Furthermore, Probe 1 has been successfully applied to detect CN^- in natural water samples. The fluorescence imaging experiments in living cells also demonstrated that Probe 1 could monitor CN^- in biological samples.