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Fermentation of the Straw Material Paja Brava by the Yeast Pichia stipitis in a Simultaneous Saccharification and Fermentation Process

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DOI: 10.4236/jsbs.2013.32014    3,660 Downloads   5,792 Views Citations

ABSTRACT

Paja Brava is a native South American grass with a high carbohydrate content. In the current work, the potential of using this feedstock for ethanol production using a simultaneous saccharification and fermentation (SSF) process with the xylose-fermenting yeast Pichia stipitis (Scheffersomyces stipitis) CBS6054 was investigated. The straw material was subjected to SO2 catalyzed steam pretreatment at 200°C and 5 min residence time, which resulted in a solubilization of pentose sugars (mainly xylose) of 64% with only minor amounts of degradation products. The obtained material, including the pretreatment liquid, was subsequently hydrolyzed and fermented in an SSF process at microaerobic conditions using either a batch or a fed-batch process at a total water-insoluble solids loading of 10%. Overall yields of ethanol based on all available sugars of 0.24 g/g and 0.27 g/g were obtained for batch and fed-batch mode of operation, respectively. The higher yield in the fed-batch process coincided with a higher degree of conversion of the sugars in the liquid medium, in particular of arabinose, for which the conversion was doubled (from 48% to 97%).


Cite this paper

C. Carrasco, H. Baudel, C. Roslander, M. Galbe and G. Lidén, "Fermentation of the Straw Material Paja Brava by the Yeast Pichia stipitis in a Simultaneous Saccharification and Fermentation Process," Journal of Sustainable Bioenergy Systems, Vol. 3 No. 2, 2013, pp. 99-106. doi: 10.4236/jsbs.2013.32014.

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