Author(s)

Emilia Heimann, Amitabh Sharma, Nalini Raghavachari, Vincent C. Manganiello, Lena Stenson, Eva Degerman

Center for Complex Network Research (CCNR), Department of Physics, Northeastern University, Boston, USA.
Department of Experimental Medical Science, Division for Diabetes, Metabolism and Endocrinology, Lund University, Lund, Sweden.
DNA Sequencing and Genomics Core, Genetics and Development Biology Center.
Pulmonary/Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health (NIH), Bethesda, USA.

Islets from RIP-PDE3B mice, exhibiting β-cell specific overexpression of the cAMP/cGMP-degrading enzyme phosphodiesterase 3B (PDE3B) and dysregulated insulin secretion, were subjected to microarray analysis. We show that osteopontin (OPN) mRNA is increased in a dose-dependent manner in islets from RIP-PDE3B mice, as compared to wild-type islets. In addition, in silico analysis shows that PDE3B and OPN are interacting. Furthermore, OPN interacts with protein kinase CK2 ina distinct submodule of the protein-protein interaction network. We studied PDE3B and OPN proteins and, in some cases, also PDE1B and PDE4C, under conditions of relevance for insulin secretion. In the presence of forskolin, PDE inhibitors, insulin, or a protein kinase CK2 inhibitor, similar alterations in protein levels of PDE3B and OPN are shown. In summary, results from using a number of strategies demonstrate a connection between PDE3B and OPNas well as a role for protein kinase CK2 inpancreatic β-cells.

Diabetes Mellitus; Pancreatic Islets; β-Cells; cAMP; Cyclic Nucleotide Phosphodiesterases; PDE; Osteopontin; Protein Kinase CK2

Heimann, E. , Sharma, A. , Raghavachari, N. , Manganiello, V. , Stenson, L. and Degerman, E. (2013) Cyclic nucleotide phosphodiesterase 3B is connected to osteopontin and protein kinase CK2 in pancreatic β-cells. Journal of Biomedical Science and Engineering, 6, 73-84. doi: 10.4236/jbise.2013.65A011.