This study investigated science anxiety of two groups of would-be science teachers. A 20-item Science Anxiety Rating Scale (SARS) adopted from Bursal (2008) was used to gather data from the selected students. 360 science education students drawn from a college of education and faculty of education of a university in South-western part of Nigeria participated in the study. The sample comprised 200 (55.56%) of the final year Bachelor’s degree in education and 160 (45.44%) of the final year Nigerian Certificate in Education students respectively. Finding of this study revealed that there was statistically significant difference in the science anxiety of the two categories of would-be science teachers.